Does PE and FITC overlap

Does PE and FITC overlap

Analysis of fluorescent protein brightness and spectral overlap.On tue, 21 sep 1999, faith kaplan wrote:Compensation levels may be set live (either manually or via software wizards) or offline.The goal of color compensation is to correctly quantify each dye withShown in red is the portion of the fitc spectrum that will be detected in the pe detector (585/40) and.Here, there is a portion of spectral overlap between the pe emission and the propidium iodide (pi) emission.

(a) representative confocal images of mk4 cells transduced with a viral vector encoding a single fluorescent protein.We've experienced this recently also.

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